Laboratory Reagent, Histological Reagent, Tissue Clearing Reagent, Visikol HISTO-1, 100mL. A simple and rapid tissue clearing formula that can be used to image tissues in 3D without damaging the underlying cellular morphology. Designed to pair with immunolabeling and reversible so that 3D histology can be followed up with traditional 2D histology. The Visikol HISTO tissue clearing approach is comprised of two different reagents called Visikol HISTO-1 and Visikol HISTO-2 whereas Visikol HISTO-1 is used first and Visikol HISTO-2 is used second. However, for small tissues such as micro-tissues and thin brain slices (500 um), only Visikol HISTO-1 is required and tissues can be imaged in Visikol HISTO-1. Larger tissues must be placed in Visikol HISTO-2 to finish clearing and then these samples can be imaged in Visikol HISTO-2.